pathway control lentivirus path-ctr5-pbs Search Results


pathway control-gfp lentivirus  (GenTarget)
90
GenTarget pathway control-gfp lentivirus
a , Experimental design schematic of LUAD cells treated with LCS3 and expression profiled by microarray and SILAC. b, Gene ontology analysis of biological processes upregulated by LCS3 determined by transcriptome and proteome profiling. Red bars signify a direct relation to redox homeostasis. c, Enrichr prediction of upstream transcription regulators of LCS3-induced RNA expression changes. d, Heatmap of NRF2 transcriptome signature in response to LCS3. e, Western blot showing induction of NRF2-regulated proteins by LCS3 and KEAP1 inactivator AI-1 (positive control). f, Schematic of antioxidant response element-green fluorescent protein <t>(ARE-GFP)</t> reporter which was transduced by <t>lentivirus</t> into H23 and H1650 cell lines. g, GFP expression of ARE-GFP relative to pathway control in response to AI-1 and LCS3. Data shown are the mean + SEM. h, Flow cytometry with oxidative stress sensor H 2 DCFDA detects induction of ROS after 90 minutes of treatment. i, Growth rescue experiment of LCS3-induced cell death in sensitive cell line H23 by antioxidants. Error bars are +/- SEM.
Pathway Control Gfp Lentivirus, supplied by GenTarget, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pathway+control+lentivirus+path-ctr5-pbs/bio_rxiv__2021__06__28__450088-279-4-7?v=GenTarget
Average 90 stars, based on 1 article reviews
pathway control-gfp lentivirus - by Bioz Stars, 2026-08
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a , Experimental design schematic of LUAD cells treated with LCS3 and expression profiled by microarray and SILAC. b, Gene ontology analysis of biological processes upregulated by LCS3 determined by transcriptome and proteome profiling. Red bars signify a direct relation to redox homeostasis. c, Enrichr prediction of upstream transcription regulators of LCS3-induced RNA expression changes. d, Heatmap of NRF2 transcriptome signature in response to LCS3. e, Western blot showing induction of NRF2-regulated proteins by LCS3 and KEAP1 inactivator AI-1 (positive control). f, Schematic of antioxidant response element-green fluorescent protein (ARE-GFP) reporter which was transduced by lentivirus into H23 and H1650 cell lines. g, GFP expression of ARE-GFP relative to pathway control in response to AI-1 and LCS3. Data shown are the mean + SEM. h, Flow cytometry with oxidative stress sensor H 2 DCFDA detects induction of ROS after 90 minutes of treatment. i, Growth rescue experiment of LCS3-induced cell death in sensitive cell line H23 by antioxidants. Error bars are +/- SEM.

Journal: bioRxiv

Article Title: A novel small molecule that induces cytotoxicity in lung cancer cells inhibits disulfide reductases GSR and TXNRD1

doi: 10.1101/2021.06.28.450088

Figure Lengend Snippet: a , Experimental design schematic of LUAD cells treated with LCS3 and expression profiled by microarray and SILAC. b, Gene ontology analysis of biological processes upregulated by LCS3 determined by transcriptome and proteome profiling. Red bars signify a direct relation to redox homeostasis. c, Enrichr prediction of upstream transcription regulators of LCS3-induced RNA expression changes. d, Heatmap of NRF2 transcriptome signature in response to LCS3. e, Western blot showing induction of NRF2-regulated proteins by LCS3 and KEAP1 inactivator AI-1 (positive control). f, Schematic of antioxidant response element-green fluorescent protein (ARE-GFP) reporter which was transduced by lentivirus into H23 and H1650 cell lines. g, GFP expression of ARE-GFP relative to pathway control in response to AI-1 and LCS3. Data shown are the mean + SEM. h, Flow cytometry with oxidative stress sensor H 2 DCFDA detects induction of ROS after 90 minutes of treatment. i, Growth rescue experiment of LCS3-induced cell death in sensitive cell line H23 by antioxidants. Error bars are +/- SEM.

Article Snippet: The ARE-GFP lentivirus and pathway control-GFP lentivirus (GenTarget, LVP981-B-PBS, Path-Ctr5-PBS) were transduced into H23 and H1650 cells with 8µg/ml polybrene (Sigma, H9268-10G) and selected with blasticidin (Life, R210-01).

Techniques: Expressing, Microarray, RNA Expression, Western Blot, Positive Control, Flow Cytometry